Early immunoreactions in peripheral blood mononuclear cells induced by puumala virus

Cvetko Krajinović, Lidija (2014) Early immunoreactions in peripheral blood mononuclear cells induced by puumala virus. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this study was to explore the components of innate and adaptive immunity important in the early peripheral immune response to hantaviral infection as well as possible regulatory effect of miRNA to peripheral immune response. Using real-time PCR array technology, the relative expression of immune response genes and miRNA was measured in peripheral blood mononuclear cells of patients with hemorrhagic fever with renal syndrome (HFRS) infected with Puumala virus (PUUV). The results showed suppression of the early immune response to PUUV i.e. genes coding for the synthesis of pattern recognition receptors, chemokines and their receptors, other cytokines, transcription factors as well as some signalling molecules. Functional analysis showed that down regulation of the expression of described set of genes modulates the inflammatory response by interfering with various cell-signalling pathways. For the first time, the biological importance of miRNA has been shown in the regulation of immune response during HFRS. Changes detected at the immune response level have been associated with disease severity but not with viral load in the blood. MAPK8, CCR5, IL-10, STAT1, STAT4, miR21 and miR19a could be potential predictors of HFRS pathogenesis.

Item Type: Thesis (Doctoral thesis)
Keywords: hantaviruses, Puumala virus, miRNA expression, immune response, hemorrhagic fever with renal syndrome
Supervisor: Markotić, Alemka and Paessler, Slobodan
Date: 2014
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 07 May 2014 11:43
Last Modified: 23 Sep 2014 08:34
URI: http://digre.pmf.unizg.hr/id/eprint/1054

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