Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)

Ecker, Christina and Forstenlechner, Peter and Milić, Dalibor and Hoffmann-Sommergruber, Karin and Cichna-Markl, Margit and Matković-Čalogović, Dubravka (2011) Expression, Purification and Crystallization of Wheat Profilin (Tri a 12). Croatica Chemica Acta, 84 (3). pp. 419-422. ISSN 0011-1643

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Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coli, purified and crystallized using the hangingdrop vapour-diffusion method. Adiffraction-data set was collected in-house from a single crystal to a resolution of 3.3 Å. The crystals belonged to space group P3221, with unit-cell parameters a = b = 58.9 Å, c = 82.5 Å, α = β = 90° and γ = 120°.

Item Type: Article
Keywords: wheat profilin, expression, crystallization, food allergy
Date: 2011
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
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Depositing User: Branka Maravic
Date Deposited: 09 May 2014 10:00
Last Modified: 09 May 2014 10:00

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