Structure of the T6 Human Nickel Insulin Derivative at 1.35 Å Resolution

Prugovečki, Biserka and Dodson, Eleanor J. and Dodson, Guy G. and Matković-Čalogović, Dubravka (2009) Structure of the T6 Human Nickel Insulin Derivative at 1.35 Å Resolution. Croatica Chemica Acta, 82 (2). pp. 433-438. ISSN 0011-1643

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This paper presents results of the structural investigation on the human insulin hexamer derivative stabilised by nickel coordination. Single crystals of the Ni-insulin derivative were prepared by the hanging drop vapour diffusion crystallisation method using metal-free insulin and nickel(II) acetate tetrahydrate. The low-temperature crystal structure was determined by the single crystal X-ray crystallographic method with data extending to 1.35 Å spacing. The investigated insulin derivative exhibits the T6 form of insulin and crystallizes in the trigonal system in space group R3, with the unit cell parameters a = b = 81.41 Å and c = 33.75 Å. There are two nickel atoms per insulin hexamer and both are octahedrally coordinated by three Nε2 atoms of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules.

Item Type: Article
Keywords: insulin derivative; nickel; X-ray structure
Date: 2009
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
Related URLs:
Depositing User: Branka Maravic
Date Deposited: 09 May 2014 13:14
Last Modified: 09 May 2014 13:14

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