Potentiometric Investigation of Specific Ionic Effects on Interactions between Bovine Serum Albumin and Weak Polyelectrolytes

Mutka, Sanela and Njegić-Džakula, Branka and Kovačević, Davor (2008) Potentiometric Investigation of Specific Ionic Effects on Interactions between Bovine Serum Albumin and Weak Polyelectrolytes. Croatica Chemica Acta, 81 (4). pp. 593-597. ISSN 0011-1643

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Abstract

The effect of salt on the behaviour of bovine serum albumin (BSA) in solution and on the interactions between BSA and the weakly charged polyelectrolytes poly(allylamine hydrochloride) and poly(dimethylaminoethylmethacrylate) was investigated by potentiometric titrations. Titrations of pure BSA solution and of the BSA solution with the addition of polyelectrolyte were performed in the presence of different salts. Three electrolytes having the same anion and a different cation were used, lithium chloride, sodium chloride, and caesium chloride. The corresponding base served as titrant. Experiments were performed at four electrolyte concentrations. The titration curves showed a strong influence of ionic strength and the electrolyte type. The cation effect was found to be in accordance with the Hofmeister series.

Item Type: Article
Keywords: potentiometric titration; bovine serum albumin; weak polyelectrolytes; ionic strength
Date: 2008
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
Related URLs:
Depositing User: mag. bibl. Ana Šafran
Date Deposited: 12 May 2014 12:03
Last Modified: 12 May 2014 12:03
URI: http://digre.pmf.unizg.hr/id/eprint/1172

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