Epitope Mapping of Macrolide Antibiotics to Bovine Serum Albumin by Saturation Transfer Difference NMR Spectroscopy

Novak, Predrag and Tepeš, Predrag and Lazić, Vedrana (2007) Epitope Mapping of Macrolide Antibiotics to Bovine Serum Albumin by Saturation Transfer Difference NMR Spectroscopy. Croatica Chemica Acta, 80 (2). pp. 211-216. ISSN 0011-1643

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Abstract

Saturation transfer difference NMR spectroscopy was employed to characterize epitopes of macrolide antibiotics, azithromycin, oleandomycin and telithromycin binding to bovine serum albumin. The structural parts of azithromycin and oleandomycin in intimate contact with bovine serum albumin were found to be similar while those of telithromycin showed similarities but also some differences. The latter were mostly due to different structural elements of antibiotics that interact with the protein, especially the alkyl-heteroaryl side chain in telithromycin and cladinose and desosamine sugars in azithromycin and oleandomycin. The epitope maps as determined in this study can contribute to better understanding of the overall bioactivity of macrolides.

Item Type: Article
Keywords: STD NMR spectroscopy; macrolide antibiotics; albumin; epitope mapping
Date: 2007
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
Related URLs:
Depositing User: mag. bibl. Ana Šafran
Date Deposited: 12 May 2014 12:11
Last Modified: 12 May 2014 12:11
URI: http://digre.pmf.unizg.hr/id/eprint/1174

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