Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions

Gruić-Sovulj, Ita and Rokov-Plavec, Jasmina and Močibob, Marko and Kamenski, Tomislav and Weygand-Đurašević, Ivana (2004) Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions. Croatica Chemica Acta, 77 (4). pp. 599-604. ISSN 0011-1643

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Abstract

Noncovalent interactions of yeast homodimeric seryl-tRNA synthetase (SerRS) and cognate tRNASer were studied by the gel mobility shift assay and zone-interference gel electrophoresis performed under the same binding and electrophoretic conditions. Purified tRNASer as well as total yeast tRNA were applied as ligands. In the absence of Mg2+, SerRS:(tRNASer)1 noncovalent complex was detected only by zone-interference gel electrophoresis. Kd values deter- mined in the presence and absence of Mg2+ were in the same range, suggesting that Mg2+ ions mainly influence dissociation-association kinetics of the complex, with a minor contribution to its thermodynamic stability. Comparison of these two assays was shown to be useful in the analysis of thermodynamic and kinetic properties of protein:nucleic acid complexes.

Item Type: Article
Keywords: aminoacyl-tRNA synthetase; gel mobility shift assay; zone-interference gel electrophoresis; SerRS:tRNASer noncovalent complexes; Mg2+ influence
Date: 2004
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
Related URLs:
Depositing User: mag. bibl. Ana Šafran
Date Deposited: 16 May 2014 10:49
Last Modified: 16 May 2014 10:49
URI: http://digre.pmf.unizg.hr/id/eprint/1406

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