Relation of osteogenic potential and stemness during mesenchymal stem cell differentiation

Brkić, Šime (2012) Relation of osteogenic potential and stemness during mesenchymal stem cell differentiation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Mesenchymal stem cells (hMSC) represent a small population of cells located in bone marrow and most of the connective tissue. hMSC are multipotent and their ability to differentiate into osteoblast makes them suitable for application in regenerative orthopedics. In this research, I investigated the level of osteogenic differentiation of hMSC after the treatmant with osteoinductive molecules under the standard protocol. In vitro differentiation was evaluated by the analysis of bone markers – alkaline phosphatase and bonesialoprotein and by analysis of markers of stemness. Oct4, Sox2 i Nanog are pluripotency markers of embryonic stem cells but their expression was also confirmed in some types of adult stem cells. The aim of this research was to investigate if they were expressed in hMSC and if their expression decreases during differentiation. Results of RT qPCR showed that Oct4, Sox2 i Nanog are expressed in undifferentiated hMSC and that their expression decreases paralel with the apperance and increase of bone markers. Immuncitochemistry analysis showed that hMSC are negative for SOX2 and NANOG proteins, but they are positive for OCT4. The level of expression of OCT4 did not change during differentiation.

Item Type: Thesis (Diploma thesis)
Keywords: mesenchymal stem cells, osteogenesis, stemness
Supervisor: Marijanović, Inga and Matić, Igor
Date: 2012
Number of Pages: 37
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 22 May 2014 13:41
Last Modified: 22 May 2014 13:41
URI: http://digre.pmf.unizg.hr/id/eprint/1609

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