Assessment of primary DNA damage in blood and different organs in swiss albino mices after exposure to ionising radiation

Modrić, Željka (2012) Assessment of primary DNA damage in blood and different organs in swiss albino mices after exposure to ionising radiation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Primary DNA damage was studied on peripheral blood and different organs in Swiss albino mice using the alkaline comet assay. The damage and repair were measured immediatelly after, 2 and 6 hours after the exposure to γ- radiation (60Co), using clinically relevant doses of 1 and 2 Gy. The majority of DNA lesions had been repaired in first 2 hours after irradiation. Brain cells processed immediately after the exposure showed more damage compared to liver and kidney cells. Dynamics of repair was estimated using cellular DNA repair index. Brain cells and peripheral leucocytes appeared to repair the damage more effectively than liver and kidney cells, suggesting higher radioresistance to administered doses. There was a significant difference in repair time and dynamics comparing the doses, especially in brain cells.

Item Type: Thesis (Diploma thesis)
Keywords: ionising radiation, comet assay, leucocytes, liver, kidney, brain, cellular DNA repair index
Supervisor: Milić, Mirta
Co-supervisor: Benković, Vesna
Date: 2012
Number of Pages: 40
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 22 May 2014 13:45
Last Modified: 19 Sep 2014 11:48
URI: http://digre.pmf.unizg.hr/id/eprint/1610

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