Liver lipid changes in green frog (Pelohylax ridibundus) during arousal from induced hibernation

Kostanjšek, Fran (2012) Liver lipid changes in green frog (Pelohylax ridibundus) during arousal from induced hibernation. Diploma thesis, Faculty of Science > Department of Biology.

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Family Ranidae is positioned in order of anurans (anura) in class of amphibians (Amphibia) and counts around 600 species world wide. In Croatia there are 7 of those species, 1 of them being included in this paper and that is Pelophylax ridibundus. Test subjects were determend using mitochondrial DNA to insure that they really are P. ridibundus since they are very similar to Pelophylax esculentus, a hybrid species between P. ridibundus and P. lessonae. Frogs were kept in the artificial hibernaculum for 30 days on a temperature of 8°C, then taken out to allow them to gradually acclimatise to temperature of 23°C. The entire procedure was recorded using IR camera due to exact temperature measurement. Liver lipids were analysed using thin layer chromatography. Blood serum lipids were also analysed for comparison to see if there is any correlation between those. Frogs were sacraficed immedietly after taking them out of hibernaculum, after 1 hour, 2 hours, 4 hours and 24 hours. Results have proven that temperature rise is in correlation with liver lipd mobilization, while that is not the case with serum lipids.

Item Type: Thesis (Diploma thesis)
Keywords: Pelophylax, hibernation, lipids, serum, liver
Supervisor: Đikić, Domagoj
Date: 2012
Number of Pages: 31
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 May 2014 11:00
Last Modified: 19 Sep 2014 11:01

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