Homologous and heterologous expression of AtBPM1 in Arabidopsis thaliana plants and in transformed Nicotiana tabacum cells

Martinčić, Jelena (2012) Homologous and heterologous expression of AtBPM1 in Arabidopsis thaliana plants and in transformed Nicotiana tabacum cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

BTB/POZ proteins are identified in many eukaryotes in which they obtain different functions. Such proteins can have some additional domains, like MATH domain. Those domains enable Arabidopsis thaliana MATH/BTB (BPM) proteins to have different protein-protein interactions. It is assumed that AtBPM1 protein participates in substrate recognition and ubiquitination in CUL3 E3 ubiquitin ligases. The exact function of AtBPM1 protein is not defined. In order to contribute to the knowledge of AtBPM1 protein function, the aim of this study was to investigate its localization in transgenic A. thaliana plants and in transformed tobacco BY-2 cells. Due to Biolistic® transformation of BY-2 cells, it is determined that AtBPM1 and ZmCUL3 proteins co-localize in cytoplasm, and partly in nucleus. AtBPM1 protein localizes to a large extent in nucleolus, where its function is probably independent of CUL3. Stable homodimers or heterodimers and ubiquitinated AtBPM1 protein is not found. Treatment with proteasome inhibitor MG132 lead to accumulation of AtBPM1 and ZmCUL3, what indicates on involvement of proteasome in degradation of examined proteins.

Item Type: Thesis (Diploma thesis)
Keywords: MATH/BTB protein domains, Arabidopsis thaliana transgenic plants, protein ubiquitination, CUL3 E3 ubiquitin ligases
Supervisor: Bauer, Nataša
Co-supervisor: Horvat, Tomislav
Date: 2012
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 May 2014 12:50
Last Modified: 27 May 2014 12:50
URI: http://digre.pmf.unizg.hr/id/eprint/1791

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