Application of fluorescence hybridization in situ in diagnosis of chronic lymphocytic leukemia

Sudar, Stanka (2012) Application of fluorescence hybridization in situ in diagnosis of chronic lymphocytic leukemia. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Chronic lymphocytic leukemia is a malignant disease of hematopoietic tissue which caused accumulation of B-lymphocytes in the bone marrow, peripheral blood, lymph nodes and others organ. It occurs more often in males than females and tends to affect older patients. The molecular cytogenetic method, fluorescence hybridization in situ (FISH), makes it possible to identify numerical and structural aberrations by using five probes labeled with specific fluorescent dyes. One hundred CLL patients were studied, 54 males and 46 females. In 68% of patients are detected chromosomal abnormalities and 32% had normal karyotypes for specific chromosomal aberrations. The most frequent aberrations are in the long arm of chromosome 13 (57%), deletions of gene p53 (24%), trisomy of chromosome 12 (22%) and finally deletions of 11q22.3 (10%). Using FISH analysis, more than 80% CLL patients show one or more cytogenetic defect and FISH is helpful in diagnosis, prognosis and evaluation of therapy for CLL.

Item Type: Thesis (Diploma thesis)
Keywords: CLL, genomic aberrations, gene ATM, gene p53
Supervisor: Marijanović, Inga
Co-supervisor: Davidović-Mrsić, Sanja
Date: 2012
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 May 2014 13:13
Last Modified: 22 Sep 2014 08:14
URI: http://digre.pmf.unizg.hr/id/eprint/1805

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