Influence of platelet lysate on the biological properties of in vitro expanded mesenchymal stem cells

Božinović, Ksenija (2012) Influence of platelet lysate on the biological properties of in vitro expanded mesenchymal stem cells. Diploma thesis, Faculty of Science > Department of Biology.

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Mesenchymal stem cells (MSCs) show immunomodulatory properties in vitro that make them interesting in the field of cellular therapy. MSCs have been clinically applied to improve engraftment in hematopoietic stem cell transplantation and to treat acute graft versus host disease. Incidence of MSCs in bone marrow (BM) is very low and in vitro expansion of MSCs is necessary. In this study MSCs derived from BM of three healthy donors were expanded in vitro. Cultivation mediums enriched with different proportion of human platelet lysate (PL) were used to expand MSCs: 10, 8, 6, 4 or 2% PL. MSCs were expanded through five passages. Cell growth kinetics, morphology and immunophenotype of expanded MSCs were analyzed. Following second passage, a small proportion of cells were used for analysis of adipogenic potential of expanded cells. During the whole period of cultivation in vitro, MSCs continuously expressed mesenchymal characteristics in all culture coditions: they adhered to plastic surface showing fibroblast shape, they expressed mesenchymal immunophenotype and differentiated into adypocites. Different content of PL in culture medium had no effect on investigated biological characteristics of the MSCs, but did have significant impact on the growth kinetics of MSC. With higher content of PL, number of harvested cells increased. Cell doubling time was inversely proportional to the content of PL in the medium.

Item Type: Thesis (Diploma thesis)
Keywords: mesenchymal stem cells, platelet lysate, bone marrow
Supervisor: Golemović, Mirna
Co-supervisor: Marijanović, Inga
Date: 2012
Number of Pages: 37
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 May 2014 08:57
Last Modified: 28 May 2014 08:57

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