Genotoxic and cytotoxic effects of cisplatin in combination with anesthetic halothane on Ehrlichov ascites tumor in mice

Šutić, Maja (2012) Genotoxic and cytotoxic effects of cisplatin in combination with anesthetic halothane on Ehrlichov ascites tumor in mice. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this study, I investigated combined effecs of cisplatin and anesthetic halotan on DNA damage in tumor cell, and their effects on irreversible cell damage leading to apoptosis in vivo. Mices used in this study were injected with 2x106 EAT cells into peritoneal cavity. Then were exposed to anesthetic halotan, cisplatin, and subjected to combined treatment of halotan after cisplatin for 3 days. Genotoxic effects of halotan on EAT cells alone, or in combined applicaton with cisplatin, was estimated by using the alkaline comet assay. The percentage of EAT cell apoptosis was estimated by flow cytometry. Halothan caused strong genotoxic effects on tumor cells in vivo, and has decreased the number of living EAT cells in peritoneal cavity. Cisplatin caused apoptosis of EAT cells (41.14%) and decreased the number of living EAT cells. Combined halothan and cisplatin increased the number of living cells in the peritoneal cavity, compared to cisplatin treatment alone.

Item Type: Thesis (Diploma thesis)
Keywords: antitumor drug, anesthesia, apoptosis, DNA damage, tumor
Supervisor: Oršolić, Nada
Date: 2012
Number of Pages: 66
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 May 2014 09:10
Last Modified: 28 May 2014 09:10
URI: http://digre.pmf.unizg.hr/id/eprint/1833

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