Heterologous expression of TaMAB2 gene in transgenic Arabidopsis thaliana

Klasić, Marija (2012) Heterologous expression of TaMAB2 gene in transgenic Arabidopsis thaliana. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

MAB proteins contain two functionally important interaction domains: MATH and BTB. It is known that MAB proteins function as a part of system by which cells eliminate unwanted proteins. Some MAB proteins (CeMEL-26, HsSPOP) are part of cullin 3 E3 ligase enzymatic complex and function as substrate adaptors. In wheat, TaMAB2 gene is specifically expressed only in fertilized egg cell, zygote and two-cell embryo. In this research heterologous expression of TaMAB2 gene in Arabidopsis was induced. High amount of TaMAB2 protein is accumulated inside the cells of transgenic plants. In transgenic plants TaMAB2 is ubiquitinated and probably assembled in homo- and heterodimers. Growth and development of transgenic plants are significantly changed regarding to wild type plants, but transgenic plants have normal phototropism and gravitropism. In BY-2 cells and onion epidermal cells TaMAB2 protein form aggregates that localizes unilaterally around nuclear envelope and near cell membrane. This results indicate on the important role of TaMAB2 in protein elimination and establishment of cell polarity during early embryogenesis of wheat.

Item Type: Thesis (Diploma thesis)
Keywords: MATH and BTB protein domains, protein degradation, CUL3 E3 ligase, fenotypical changes, fototropism, gravitropism, subcellular localization, transgenic Arabidopsis thaliana
Supervisor: Bauer, Nataša
Co-supervisor: Juranić, Martina
Date: 2012
Number of Pages: 72
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 May 2014 09:14
Last Modified: 28 May 2014 09:14
URI: http://digre.pmf.unizg.hr/id/eprint/1834

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