Epigenetic regulation of surface N-glycosylation in HeLa cells

Barišić, Darko (2012) Epigenetic regulation of surface N-glycosylation in HeLa cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

N-glysoylation is a covalent modification of a protein important for its proper folding and function. Since cell surface represents the first front in cell-to-cell communication and communication between the cell and its environment, surface glycosylation can reflect changes in the environment as well as modify cellular signaling, recognition and adaptive changes in reaction to pathogens and factors of immune system. Changes in expression of glyco-genes through variety of epigenetic mechanisms represent one aspect of regulating glycan synthesis and protein glycosylation. To address the questions regarding the role of epigenetic information in N-glycoslylation, we studied changes in surface N-glycome of HeLa cells upon treatment with epigenetic inhibitors of DNA methyltransferases and histone deacetylasesas as well as reversibility of altered N-glycan profile. Since epigenetic inhibitors are currently explored as therapeutics in treatment of cancer, the presented work contributes to our understanding of their efficiency in altering the N-glycan profile of cancer cells.

Item Type: Thesis (Diploma thesis)
Keywords: DNA methylation, epigenetic inhibitors, HeLa cell line, histone acetylation, protein N-glycosylation, surface N-glycome
Supervisor: Zoldoš, Vlatka
Co-supervisor: Horvat, Tomislav
Date: 2012
Number of Pages: 56
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 May 2014 09:17
Last Modified: 28 May 2014 09:17
URI: http://digre.pmf.unizg.hr/id/eprint/1835

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