Genome changes in atypic cells in urine proved by fluorescent in situ hybridization method

Jurković, Sven (2012) Genome changes in atypic cells in urine proved by fluorescent in situ hybridization method. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Bladder cancer is one of the leading carcinomas in male population. It is possible to detect genome changes in urine sediment cells by highly sensitive, specific and noninvasive molecular biology methods like interphase-FISH (fluorescent in situ hybridization). The aim was to examine and prove genome changes in atypic cells in urine. Structural and numeric chromosomal aberrations were detected by interphase-FISH method, which uses four labeled probes which hybridize to centromeric regions of chromosome 3 (CEP 3), chromosome 7 (CEP 7) and chromosome 17 (CEP 17), and 9p21 locus (LSI p16). 50 samples of urine of patients were analysed. Positive result was found in 21 (42%) patients. In 5 (24%) patients there were just numeric, in 15 (71%) patients just structural, and in 1 (5%) patient both structural and numeric chromosomal aberrations. The conclusion is that familiar genome changes in bladder cancer cells can be detected and monitored by interphase-FISH method.

Item Type: Thesis (Diploma thesis)
Keywords: bladder cancer, sensitivity, specificity, chromosomal aberrations
Supervisor: Marijanović, Inga
Date: 2012
Number of Pages: 47
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 03 Jun 2014 12:01
Last Modified: 24 Sep 2014 09:13
URI: http://digre.pmf.unizg.hr/id/eprint/2160

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