Changes of oxidative stress biomarkers in organs of immune system in mice exposed to imazalil, cypermethrin and carbendazim

Cvitanović, Antonija (2012) Changes of oxidative stress biomarkers in organs of immune system in mice exposed to imazalil, cypermethrin and carbendazim. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Imazalil, cypermethrin and carbendazim are detected in plants for human nutrition. To explore whether their combinations, applied orally in low doses, would induce immunotoxicity, a subchronic in vivo experiment was conducted. Doses of 10 mg/kg of imazalil and cypermetrin and 20 mg/kg of carbendazim and their combinations were given to Swiss mice daily during 28 days. After 24 hours from the last dose, oxidative stress biomarkers in immune system organs were analysed: catalase (CAT) and superoxide dismutase (SOD) activities, malonilaldehyde (MDA), 8-iso prostaglandin F2α and glutathione (GSH) concentrations. Compared to control group these markers changed their activities and concentrations. Our results suggested that these pesticides have the potential to induce oxidative stress of the immune system. Decreases in GSH concentrations were determined in all groups except for groups with carbendazim in thymus and lymph node. A general increase in malonilaldehyde (MDA) concentration was observed in all test groups, which suggested that the toxic effects of these pesticides were probably exerted through free radical generation. Increases in superoxide dismutase and catalase activities in spleen point to the excessive production of reactive oxygen species.

Item Type: Thesis (Diploma thesis)
Keywords: catalase, superoxid dismutase, malonilaldehyd, 8-iso prostaglandin F2α, glutathion, immunotoxicity
Supervisor: Đikić, Domagoj
Date: 2012
Number of Pages: 67
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 03 Jun 2014 13:02
Last Modified: 19 Sep 2014 09:36
URI: http://digre.pmf.unizg.hr/id/eprint/2180

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