Inhibition of urokinase plasminogen activation system in breast cancer cells

Majstorović, Ivana (2012) Inhibition of urokinase plasminogen activation system in breast cancer cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Urokinase plasminogen activation system is a multi-component regulatory system that plays a key role in many physiological and pathological processes, mainly by extracellular proteolysis. It consists of urokinase plasminogen activator, its receptor and two inhibitors. The aim of this thesis was to investigate the effects of sodium salicylate, an active aspirin metabolite, on MDA MB-231 morphology and basal urokinase plasminogen activator activity. Zymography and caseinolysis following cell treatment with salicylate revealed a concentration and time dependant decrease in urokinase activity. While RT PCR revealed no significant changes in transcription levels of urokinase, its receptor, and inhibitor, Western blot showed a mild decrease in urokinase expression. Analysis of protein extracts indicated a slight change of NF-κB expression, and RT PCR showed decrease in integrin αv and β5 mRNA. It was concluded that sodium salicylate caused decrease in uPA activity and integrin expression.

Item Type: Thesis (Diploma thesis)
Keywords: uPA (urokinase-type plasminogen activator), sodium salicylate, MDA MB-231 cell line uPAR (uPA receptor), PAI-1 (plasminogen inhibitor type 1), integrin αv, integrin β5
Supervisor: Matulić, Maja
Date: 2012
Number of Pages: 37
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 04 Jun 2014 10:27
Last Modified: 04 Jun 2014 10:27
URI: http://digre.pmf.unizg.hr/id/eprint/2201

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