Effects of abiotic stress on level of global genome and specific rDNA methylation in Quercus robur (L.) and Lilium bosniacum (Beck) Beck ex Fritsch)

Bečeheli, Ivona (2012) Effects of abiotic stress on level of global genome and specific rDNA methylation in Quercus robur (L.) and Lilium bosniacum (Beck) Beck ex Fritsch). Diploma thesis, Faculty of Science > Department of Biology.

[img] PDF
Restricted to Registered users only
Language: Croatian

Download (2MB) | Request a copy

Abstract

Plants, as sessile organisms that can not avoid stress, have developed an effective short-term strategies for adaptation to changes in the environment. These strategies imply rapid changes in gene expression through epigenetic mechanisms. This thesis deals with the study of epigenetic changes in genomes of two species, the common oak and Bosnian lily, growing in habitats continuously exposed to abiotic stress. The level of total genomic DNA methylation and methylation of rDNA region was analysed using method CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification). CRED-RA revealed statistically significant difference in methylation pattern between the population of Bosnian lily expossed to stres and the control population. Due to extremely high intra-population genetic diversity of the common oak, CRED-RA method proved to be insufficiently precise to determine methylation events on population level. A higher frequency of RAPD polymorphisms was recorded in populations exposed to stress comparing to control populations of both species. Analysis of specific rDNA methylation revealed hypermethylation of coding regions and hypomethylation of intergenic region of oak rDNA. Comparing the CRED-RA pattern and restriction maps for 18S-26S rRNA subunit of this species, 4 hypomethylated loci were found within the intergenic spacer, which implies on the possible role in regulation of rRNA gene transcription.

Item Type: Thesis (Diploma thesis)
Keywords: CRED-RA, IGS, methylation, polymorphism, RAPD
Supervisor: Zoldoš, Vlatka
Date: 2012
Number of Pages: 90
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 04 Jun 2014 11:06
Last Modified: 04 Jun 2014 11:06
URI: http://digre.pmf.unizg.hr/id/eprint/2202

Actions (login required)

View Item View Item