The combined effect of anaesthetic sevoflurane and ionising radiation on primary DNA damage in mice

Halovanić, Sanja (2012) The combined effect of anaesthetic sevoflurane and ionising radiation on primary DNA damage in mice. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Sevoflurane (hexafluoroisopropyl fluoromethyl ether) is inhalation anaesthetic which low solubility and non-irritability, provides its wide application in induction and maintenance of general anesthesia. Ionising radiation is widely used in medicine (radiotherapy). The aim of the study was to determine and compare the interaction of γ-radiation (60Co) in a clinically relevant dose (1 Gy) and anaesthetic sevoflurane (2.4%, 50:50) in the model of Swiss albino mices, with an emphasis on the study of the degree of sensitivity of different types of tissue (brain, liver and kidneys), and peripheral blood leukocytes. For the assessment of primary DNA damage, the method of alkaline comet assay was applied immediately after, and 2, 6 and 24 h of exposure to radiation and anaesthesia. The strongest genotoxic effect was measured in kidney and brain cells 2 hours, in the blood 6 hours, and in the liver 24 hours after the treatment of the animals as compared to a control group. To test the difference in the amount of repair between blood and various tissues, cellular DNA repair index (CRI) was calculated, which showed different sensitivity of organs.

Item Type: Thesis (Diploma thesis)
Keywords: genotoxicity, comet assay, leukocytes, liver, kidney, brain, cellular DNA repair index
Supervisor: Benković, Vesna
Date: 2012
Number of Pages: 68
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 06 Jun 2014 07:39
Last Modified: 19 Sep 2014 10:19
URI: http://digre.pmf.unizg.hr/id/eprint/2276

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