Cytogenetic effects of bee venom on human lymphocytes

Tomičić, Lucija (2011) Cytogenetic effects of bee venom on human lymphocytes. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Western medicine is turning more and more towards natural products as potential solutions for the health problems that occur in human's population. Bee venom is one of those products. It has great potential for chronic pain relief and it is used for treatment of rheumatic diseases, neurological diseases and various dermatological conditions and also for treatment of tumors. The aim of this study was to assess the effect of high concentration of bee venom (100 μg/ml) on human peripheral blood in vitro over a range of time spans (from 10 min to 24 h). Parameters of comet assay showed genotoxic effect of bee venom on human peripheral blood lymphocytes in comparison with control in time period of 30 min, 6 and 24 h for tail length and of 6 and 24 h for tail moment and tail intensity. Results of micronucleus test also showed genotoxic effect of high concentration of bee venom on human peripheral blood lymphocytes in comparison with control. Method parameters (number of micronucleus, nuclear buds and nucleoplasmic bridges) showed statistically significant increase (p<0,05) for all lengths of exposure in comparison with control, therefore time dependence was established. Results obtained by this study indicate that high concentration of bee venom can lead to cellular instability. Future research should be focused on deeper understanding of the mechanism of action of bee venom and its components on human cells and finding the best possible application of this natural product in medicine.

Item Type: Thesis (Diploma thesis)
Keywords: bee venom, human peripheral blood lymphocytes, DNA damage, comet assay, micronucleus assay
Supervisor: Garaj Vrhovac, Vera
Date: 2011
Number of Pages: 71
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 09 Jun 2014 08:35
Last Modified: 16 Feb 2016 13:55
URI: http://digre.pmf.unizg.hr/id/eprint/2298

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