Expression of proinflammatory and proapoptotic genes during the chronic phase of recovery after ischemic injury in mouse brain

Zirdum, Domagoj (2011) Expression of proinflammatory and proapoptotic genes during the chronic phase of recovery after ischemic injury in mouse brain. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Stroke is the second most common cause of death in the world and one of the most frequent causes of disability in humans. Recent research discovered that TLR2, a member of Toll-like receptor protein family, is one of the most important triggers of inflammation after ischemic injury. The hypothesis was that the expression of proinflammatory cytokines decreases and those of proapoptotic factors increases in TLR2 knockout mice during the chronic phase of recovery after ischemic brain injury. The aim was to show how gene expression changes during the 1 month period after ischemia. Middle cerebral artery occlusion (MCAO) was perfomed to induce ischemia in mouse brain and qPCR to measure the relative expression of proinflammatory and proapoptotic genes. The results showed a decrease in mRNA levels of proinflammatory cytokines and disabled TLR2 mRNA and an increase in mRNA levels of proapoptotic factors in TLR2 KO during the 1 month period after ischemia. Interaction between the apoptotic and the inflammatory signaling pathway could provide insight in evaluating anti-inflammatory therapies for stroke.

Item Type: Thesis (Diploma thesis)
Keywords: TLR2, stroke, mouse, ischemia, cytokines, inflammation, apoptosis
Supervisor: Gajović, Srećko
Date: 2011
Number of Pages: 75
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Jun 2014 08:30
Last Modified: 18 Sep 2014 11:14
URI: http://digre.pmf.unizg.hr/id/eprint/2312

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