Glycosylation analysis of immunoglobulin G in inflammatory bowel disease by liquid chromatography

Trbojević Akmačić, Irena (2015) Glycosylation analysis of immunoglobulin G in inflammatory bowel disease by liquid chromatography. Doctoral thesis, Faculty of Science > Department of Chemistry.

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Abstract

Inflammatory bowel disease is chronic inflammatory disorder of the gastrointestinal tract which is considered to be the result of the interaction of genetic and environmental factors. Genetic studies have shown pleiotropy between IgG glycosylation and inflammatory bowel disease. IgG N-glycosylation affects its structure and immune function. IgG N-glycosylation in 804 inflammatory bowel disease patients and healthy controls was analyzed within this dissertation, which makes it the largest study of glycans in inflammatory bowel disease. The results demonstrated statistically significant decrease in IgG galactosylation in Crohn's disease and ulcerative colitis compared to healthy controls, as well as statistically significant decrease in IgG monosialylation in Crohn’s disease. Increase in proportion of structures with bisecting GlcNAc was observed in inflammatory bowel disease patients compared to healthy controls. Therefore, IgG has a potential as diagnostic and prognostic biomarker for inflammatory bowel disease. Within this dissertation HILIC-UPLC method was validated, and new high-throughput preparation method for N-glycan analysis by liquid chromatography was developed, optimized, and validated. Improved methodology will enable more accurate and informative N-glycan analysis in the future.

Item Type: Thesis (Doctoral thesis)
Keywords: Crohn's disease, HILIC, immunoglobulin G, inflammatory bowel disease, liquid chromatography, N-glycosylation, ulcerative colitis
Supervisor: Lauc, Gordan
Date: 2015
Number of Pages: 175
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Branka Maravic
Date Deposited: 07 Dec 2015 15:30
Last Modified: 07 Dec 2015 15:32
URI: http://digre.pmf.unizg.hr/id/eprint/233

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