Expression of genes involved in TLR2 signaling pathway during the chronic phase of recovery after ischemia in mouse brain

Krmpotić, Lora (2011) Expression of genes involved in TLR2 signaling pathway during the chronic phase of recovery after ischemia in mouse brain. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

15 million people a year worldwide are afflected with stroke which is the main cause of disability in adults. Ischemic stroke is caused by blockage of blood flow in the brain, which subsequently causes hyperfusion, excitotoxicity, oxydative stress, damage of blood-brain barrier and postischemic inflammation. The aim of the thesis was to show how the expression of TLR2 signaling pathway genes changes during the chronic phase of recovery, 72 h to 1 month after induced ischemia in mouse brain. Middle cerebral artery occlusion (MCAO) was performed on mice for induction of ischemia and qPCR to measure the relative expression of selected genes. The results showed that the expression of pathway activators decreased and inhibitors increased in TLR2 KO relative to WT, in chronic phase of reperfusion. Regulation of these genes in TLR2 signaling pathway could prove to be paramount in attenuating damage caused by ischemic stroke.

Item Type: Thesis (Diploma thesis)
Keywords: TLR2, stroke, mouse, ischemia, inflammation
Supervisor: Gajović, Srećko
Date: 2011
Number of Pages: 83
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 11 Jun 2014 07:53
Last Modified: 11 Jun 2014 07:53
URI: http://digre.pmf.unizg.hr/id/eprint/2356

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