Relation of DNA traces from different surfaces with donor age and gender

Markulin, Dora (2011) Relation of DNA traces from different surfaces with donor age and gender. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this research was to discover the dependence of trace DNA quantity on various factors. We analyzed the correlation between handling time, donor age and gender and quantity of DNA trace left by individuals on items of different textures (paper, plastic, plastic coated metal) during rubbing with fingers and palm or holding items in a hand. Items were swabbed with moistened cotton swab, DNA was isolated by Chelex method and quantified by Real Time PCR. The results showed the amount of DNA transferred to an item was independent of the handling time. In contrast, it was dependent of item texture where the greatest amount was left on plastic coated metal and the least on paper. The greatest amount of DNA trace was left by individuals ranging in age from 35 to 44 years and men. Finally, this study showed that the tendency to deposit DNA on an item was an independent characteristic of a person.

Item Type: Thesis (Diploma thesis)
Keywords: forensic analysis, DNA transfer, shedding, palm epithelium, cell-free DNA, paper, plastic
Supervisor: Marijanović, Inga
Co-supervisor: Gršković, Branka
Date: 2011
Number of Pages: 58
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 12 Jun 2014 09:07
Last Modified: 12 Jun 2014 09:07
URI: http://digre.pmf.unizg.hr/id/eprint/2365

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