Cell-stroma interacting genes in patients with primary myelofibrosis

Livun, Ana (2013) Cell-stroma interacting genes in patients with primary myelofibrosis. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Primary myelofibrosis (PMF) is rare and aggressive myeloproliferative neoplasia. PMF is characterized by bone marrow (BM) fibrosis, leukoerythroblastosis, teardrop poikilocytosis, extramedullary hematopoesis and elevated number of hematopoietic stem CD34+ cells (HSCs) in the peripheral blood (PB). To address if change in expression of HSCs-stromal interacting genes is present, expression of SPARC and CXCR4 were measured in BM and PB mononuclear cells and cultured stromal cells from 32 patients. This investigation also included genes involved in metabolism (COX-2), transcription (HIF1α), differentiation, signaling and proliferation (PAX5 C terminus, FOS, K-RAS). Patients treated with lenalidomide/prednisone were monitored during a 2 year period. Those patients haven’t show any change, but changes in expression are visible for SPARC, K-RAS, CXCR4, PAX5 C terminus and HIF1α gene in BM and PAX5 C terminus in PB compared to healthy individuals. Stromal cells show a wide range of changes.

Item Type: Thesis (Doctoral thesis)
Keywords: CD34+cells, CXCR4, lenalidomide, stroma, prednisone, primary myelofibrosis, SPARC
Supervisor: Kušec, Rajko
Date: 2013
Number of Pages: 81
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 24 Jan 2014 11:05
Last Modified: 22 Sep 2014 12:13
URI: http://digre.pmf.unizg.hr/id/eprint/240

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