Mechanisms of antibiotic resistance in Gram-negative bacteria from coastal area of the Kaštela Bay

Maravić, Ana (2013) Mechanisms of antibiotic resistance in Gram-negative bacteria from coastal area of the Kaštela Bay. Doctoral thesis, Faculty of Science > Department of Biology.

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To understand the role of polluted marine environment in dissemination of human pathogenic bacteria resistant to antibiotics, we explored the incidence and mechanisms of resistance in bacterial communities from coastal area of the Kaštela Bay. From 2008 to 2010 2,569 isolates were isolated from seawater and fauna, identified and determined their phenotypic susceptibility to antibiotics. Characterization of resistance genes, with focus on extended spectrum-β-lactamases, were performed by PCR, sequencing, conjugation and Southern blot hybridization, while genetic relationship of marine isolates and isolates from Split University Hospital were examined using RAPD and PFGE genotyping. TEM, SHV and CTX-M β-lactamases were identified in a significant percent (4.7%) of Enterobacteriaceae, Aeromonas, Pseudomonas, Burkholderia, Stenotrophomonas and Vibrio isolates. Molecular analyses indicated that enterobacteriaceae as human commensals play a significant role in the transmission of genes, specifically CTX-M β-lactamases, from marine isolates to human pathogens, which is of great importance in understanding the epidemiology of resistance genes and, thus, the potential consequences of faecal pollution on human health.

Item Type: Thesis (Doctoral thesis)
Keywords: resistance to antibiotics, Gram-negative bacteria, β-lactamases, ESBL; marine environment; Kaštela Bay
Supervisor: Skočibušić, Mirjana
Date: 2013
Number of Pages: 123
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 24 Jan 2014 11:39
Last Modified: 22 Sep 2014 12:17

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