The effect of iron on the glycogen content in the carp liver (Cyprinus carpio L.)

Plačko, Ema (2011) The effect of iron on the glycogen content in the carp liver (Cyprinus carpio L.). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

One of today's major concerns is heavy metal pollution of water. Heavy metals are dangerous because of their persistency, high toxicity and tendency to accumulate in the environment. In this way, iron is also a potential toxin if its amount in the organism increases, even though it is one of the essential metals. Increased accumulation of iron in the organism affects a number of metabolic paths, including carbohydrate metabolism. Therefore, the aim of this research was to monitor the effect of iron (Fe2+ and Fe3+) on the glycogen content in the carp liver (Cyprinus carpio L.) during long-term exposure (105 days) to maximum allowed waterborne iron concentration (1 mg/L). The glycogen content in the liver was visualized by histochemical PAS reaction and then analyzed by light microscopy (Nikon Eclipse E600) and a morphometrical computer program (Lucia G 4.80). The results show that during the exposure glycogen content decreases as a response to the environmental stress of heavy metal pollution. Iron also damages the blood vessel walls and affects the hepatocyte size. Regarding the given results it can be concluded that the maximum allowed waterborne iron concentration, through a longer period, can cause significant histomorfological and biochemical changes in the liver.

Item Type: Thesis (Diploma thesis)
Keywords: carp, Cyprinus carpio L., hepatopancreas, iron, glycogen, histochemistry, alcian – PAS method
Supervisor: Lacković Venturin, Gordana
Date: 2011
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 08 Jul 2014 07:25
Last Modified: 16 Feb 2016 14:03
URI: http://digre.pmf.unizg.hr/id/eprint/2464

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