Analysis of grapevine intravarietal polymorphism using retrotranspone-based markers

Rajič, Jelena (2011) Analysis of grapevine intravarietal polymorphism using retrotranspone-based markers. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Grapevine (Vitis vinifera sp.) is one of the most important fruit cultures represented with approximately 10 000 different varieties which are constituted of numerous clones. Clonal selection of grapevine singles out those clones that show significant deviations in phenotype, resulting from some genetic changes between these clones. Possible explanations of clonal variability are the following: polyclonal origin of cultivars, phatogen infections, somatic mutations, epigenetics and chimerism. In this work we studied clonal variation of the Croatian autochthonous cultivar Plavac mali by REMAP and IRAP techniques as well as genetic basis of skin color changes in Plavac mali sivi. These techniques use insertions of retrotransposons as a source of DNA polymorphisms. Both are based on polymerase chain reaction (PCR), which is used for amplification retrotransposon insertion sites in genomic DNA. Detected polymorphisms could be used for developing DNA markers for early identification of clones with useful / superior agronomic traits. A relatively small number of polymorphisms are detect between the clones, which could only partially be correlated with their expressed phenotypic variation. However, applied techniques have proven to be fast, easy and cheap way to preliminary distinguish grape varieties.

Item Type: Thesis (Diploma thesis)
Keywords: grapevine, retrotransposons, IRAP, REMAP
Supervisor: Besendorfer, Višnja
Co-supervisor: Malenica, Nenad
Date: 2011
Number of Pages: 55
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Jul 2014 11:38
Last Modified: 10 Jul 2014 11:38
URI: http://digre.pmf.unizg.hr/id/eprint/2486

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