The effect of heat stress on the genetic stability of tobacco Nicotiana tabacum L.

Debogović, Lea (2011) The effect of heat stress on the genetic stability of tobacco Nicotiana tabacum L.. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Climate change is one of the biggest challenges we face today. Plants are sessile organisms, and as such do not have the ability to regulate their temperature by behavior. Therefore, stressful environmental conditions as a result of global warming may have catastrophic consequences for plants. The objective of this research was to determine the effect of heat stress on the genetic stability of tobacco plants. Plants of different ages, one and three months, were exposed short-term (2 h and 4 h) to temperature of 42 °C and left to recover for 3 days. The extent of DNA damage and DNA alterations after the treatment and during the recovery period were evaluated using the Comet assay and RAPD. Results of the Comet assay showed statistically significant damage to DNA immediately after 2 h and 4 h of exposure to high temperature compared to the control sample, but they also showed that damage was repaired after the 3 days of recovery, regardless of the plant age. RAPD analysis showed no difference in DNA profiles.

Item Type: Thesis (Diploma thesis)
Keywords: heat stress, genotoxicity, comet assay, DNA polymorphism, oxidative stress, tobacco
Supervisor: Pavlica, Mirjana
Date: 2011
Number of Pages: 40
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Jul 2014 12:05
Last Modified: 01 Oct 2014 09:59
URI: http://digre.pmf.unizg.hr/id/eprint/2492

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