Effect of copper on the genotoxicity of cadmium in tobacco, Nicotiana tabacum L.

Ćetković, Maja (2011) Effect of copper on the genotoxicity of cadmium in tobacco, Nicotiana tabacum L.. Diploma thesis, Faculty of Science > Department of Biology.

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Rapid industry development causes increase of environmental pollution. Among numerous environmental contaminants, heavy metals are of significant value. Toxicity of heavy metals depends on their concentration, mobility in the plant and environmental conditions. Essential metals such as copper, when present in excessive concentration become toxic to plants. Cadmium is one of the nonessential toxic heavy metals that is genotoxic and mutagenic to organisms. The main goal of this study was to determine the sensitivity of the tobacco genome on the heavy metals copper and cadmium, individually and in mixture by comet assay and to investigate whether copper has antagonistic or synergistic effect on the cadmium genotoxicity. Bioaccumulation of heavy metals was measured with inductively coupled plasma atomic emission spectroscopy. Analysis of metal content showed that cadmium accumulation was higher in roots than in leaves of tobacco plants. Comet assay confirmed the sensitivity of the tobacco genome to cadmium and copper. Cellular comet assay showed antagonistic effect of copper on cadmium genotoxicity in leaves, and synergistic effect in roots. Acellular comet test showed that copper reduces genotoxic effects of cadmium in leaves.

Item Type: Thesis (Diploma thesis)
Keywords: Nicotiana tabacum L., copper, zinc, cadmium, genotoxicity, comet assay, bioacumulation
Supervisor: Pavlica, Mirjana
Date: 2011
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Jul 2014 12:12
Last Modified: 18 Sep 2014 11:24
URI: http://digre.pmf.unizg.hr/id/eprint/2494

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