The role of RSC complex in chromatin structure remodeling of the yeast Saccharomyces cerevisiae PHO gene promoters

Musladin, Sanja (2014) The role of RSC complex in chromatin structure remodeling of the yeast Saccharomyces cerevisiae PHO gene promoters. Doctoral thesis, Faculty of Science > Department of Chemistry.

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Abstract

The yeast S. cerevisiae PHO5 promoter has proven an xcellent model for studying the regulation of gene expression through chromatin structure remodeling. The complete set of yeast remodeling complexes non-essential for cell viability was tested for involvement in massive transition of promoter chromatin structure upon induction, but none of the many tested remodeler gene mutations prevented PHO5 promoter opening. The role of RSC, only remodeling complex essential for cell viability, in chromatin remodeling upon induction of PHO promoters was unassessed in vivo so far. Here, the involvement of RSC complex was examined and it was shown that complete inactivation of RSC’s activity prevented chromatin transition at the PHO5 promoter. A set of control experiments confirmed a direct effect of RSC inactivation, at the level of promoter chromatin remodeling, rather than being a consequence of compromised signaling pathway or cell cycle arrest. Interestingly, RSC was hardly involved in chromatin transitions at the PHO8 and PHO84 promoters, despite cofactor recruitment by the same transactivator.

Item Type: Thesis (Doctoral thesis)
Keywords: chromatin remodeling, PHO promoters, RSC complex, transcriptional regulation, yeast Saccharomyces cerevisiae
Supervisor: Barbarić, Slobodan
Date: 2014
Number of Pages: 111
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: mag. bibl. Ana Šafran
Date Deposited: 18 Jul 2014 11:38
Last Modified: 18 Apr 2016 14:31
URI: http://digre.pmf.unizg.hr/id/eprint/2523

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