The influence of Puumala virus on the micro RNA expression in human epithelial kidney cells

Kurolt, Ivan-Christian (2014) The influence of Puumala virus on the micro RNA expression in human epithelial kidney cells. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

This study investigates the expression or secretion of selected microRNAs (miRNAs) in vitro in HEK293 cells infected with Puumala virus (PUUV) or transiently transfected with PUUV N protein, and ex vivo in urine samples of patients with hemorrhagic fever with renal syndrome (HFRS) infected with PUUV, using real-time PCR. Limited deregulation of miR-21 and miR-27a expression was detected in HEK293, while miRNA abundance in supernatants was aberrant. Contrary to our hypothesis, transient expression of PUUV N did not yield significant changes in miRNA expression. In urinary samples of HFRS patients and patients with acute pyelonephritis, miR-21 and miR-27a were more abundant, while let-7e could be HFRS specific. In mild patients with HFRS only miR-21 and let-7e were deregulated, whereas in severe patients miR-24, miR-27a, and miR-127 were also altered. Detected miRNAs could present HFRS biomarkers correlating significantly with urea and creatinine levels, which represent hallmarks of HFRS progression and severity.

Item Type: Thesis (Doctoral thesis)
Keywords: hantaviruses, Puumala virus, miRNA expression, hemorrhagic fever with renal syndrome, acute pyelonephritis, renal disease
Supervisor: Markotić, Alemka
Co-supervisor: Paessler, Slobodan
Date: 2014
Number of Pages: 122
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 22 Jul 2014 09:30
Last Modified: 24 Sep 2014 08:21
URI: http://digre.pmf.unizg.hr/id/eprint/2532

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