Evaluation of DNA damage after applying Cypermethrine in Swiss albino mice

Mihaljević, Josipa (2011) Evaluation of DNA damage after applying Cypermethrine in Swiss albino mice. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Cypermethrin belongs to the pyrethroid group of insecticides that are relatively new group of pesticides that are increasing in use. It is used to control many pests of fruits and vegetables, crops, including landscape maintenance. It acts as a neurotoxin and thereby suppresses insects. The effects of cypermethrin were studied by applying in vivo micronucleus test on peripheral blood reticulocytes and alkaline comet assay on blood cells, spleen, lymph nodes and thymus of Swiss albino mouse. Plan of the experiment was consistent with the provisions of the OECD 407th. During the period of 28 days, mice were receiving the corresponding NOAEL dose (10 mg/kg) for cypermethrin which is 1 / 100 LD50 lethal dose. The results were compared with the control group. Micronucleus test results show that peripheral blood reticulocytes in male and female mice are equally susceptible to exposure to cypermethrin. Comet assay results show that the various different types of cells of mice are sensitive to exposure to cypermethrin, with the most sensitive leukocytes in male mice and spleen cells in female mice.

Item Type: Thesis (Diploma thesis)
Keywords: cypermethrine, micronucleus test, comet assay, genotoxicity
Supervisor: Benković, Vesna
Date: 2011
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 01 Sep 2014 11:42
Last Modified: 18 Sep 2014 09:43
URI: http://digre.pmf.unizg.hr/id/eprint/2568

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