Intracellular trafficking of solid lipid nanoparticles

Herceg, Viktorija (2011) Intracellular trafficking of solid lipid nanoparticles. Diploma thesis, Faculty of Science > Department of Biology.

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Solid lipid nanoparticlers (SLN) are colloidal drug carriers made of biodegradable lipids. They can improve the bioavailability of poorly water-soluble molecules and provide sustained release over time. SLN loaded with the anti-cancer drug edelfosine showed promising results in in vivo xenograft animal models. However, edelfosine mechanism of action remains unknown. The objectives were: to elucidate the uptake and intracellular trafficking of SLN by using fluorescent microscopy and transmission electron microscopy (TEM) and to evaluate the effect of free edelfosine and edelfosine-loaded SLN on cancer cell lines by inhibition of proliferation and apoptosis. SLN were prepared by hot homogenization. Various fluorescent compounds (Perylene, DiD, Rhodamine, Nile Red) were successfully encapsulated in SLN. SLN were uptaken by HL-60, K562 and GS-9L cells as demonstrated by fluorescent and TEM studies. GS-9L cell were resistant to action of free edelfosine as demonstrated by inhibition of proliferation. Edelfosine loaded-SLN were effective in treating GS-9L cell line whereas the free-drug was practically devoid of efficacy.

Item Type: Thesis (Diploma thesis)
Keywords: solid lipid nanoparticles, edelfosine, Nile Red, fluorescence microscopy, transmission electron microscopy, inhibition of proliferation, apoptosis
Supervisor: Blanco-Prieto, María J.
Date: 2011
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 01 Sep 2014 12:35
Last Modified: 18 Sep 2014 08:47

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