Molecular detection of citrus variegation virus

Sarađen, Željka (2011) Molecular detection of citrus variegation virus. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The purpose of this study was to determine the presence of Citrus variegation virus (CVV) from field samples and its possible distribution in Croatia using laboratory methods based on the detection of viral capsid protein gene. Citrus samples of various origines were included: from commercial orchards, gardens and citrus collection planting of the Institute for Adriatic Crops and Karst Reclamation in Split. Conducted molecular detection of CVV was the first study of that kind in our country and it was necessary to find the most appropriate detection methods and optimize the protocols. For the detection of CVV molecular methods RT-PCR and IC/RT-PCR were applied, while for the nucleic acids isolation RNeasy Plant Mini Kit and the procedure described in Bennani et al. (2002) were evaluated. The methodology using extraction procedure by Bennani et al. including the amplification of CVV coat protein gene by RT-PCR proved to be adequate and cost effective method for this purpose. Additional improvements were made by introducing. Tris-free buffer system (Brody and Kern, 2004) that is also safer for the environment. All tested samples of citrus collected here were negative for the presence of CVV.

Item Type: Thesis (Diploma thesis)
Keywords: CVV, citrus, RT-PCR, IC/RT-PCR
Supervisor: Škorić, Dijana
Date: 2011
Number of Pages: 37
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 01 Sep 2014 12:48
Last Modified: 01 Sep 2014 12:48
URI: http://digre.pmf.unizg.hr/id/eprint/2576

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