Application of induction factor in comparison of the results obtained in comet assay under different conditions of electrophoresis

Urošević, Sonja (2010) Application of induction factor in comparison of the results obtained in comet assay under different conditions of electrophoresis. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Comet assay is a sensitive, quick and inexpensive method that detects DNA damage in single cells. Because of this, it is often used in the assessment of genotoxic effects. Different conditions and ways of implementation of Comet assay have made it difficult to compare given data and results. Thus, the aim of this research is to investigate the standardization that could enable comparison of results given by Comet assay performed in different electrophoretic conditions. In this study, two different cell lines were used – PLHC-1 fish hepatoma cell line and ZFL cell line, as well as two genotoxic agens - ethyl methanesulfonate (EMS) and hydrogen peroxide (H2O2). Comet assay was performed for all given variables and in 4 different conditions of electrophoresis, depending on intensity (25-35 V) and duration (15-20 min). Observed genotoxic effect was demonstrated as percentage of DNA that migrated in the tail during the electrophoresis (% tDNA) for both cell lines treated with EMS and H2O2. Induction factor (IF) was represented as difference in % tDNA between the control and treated groups.

Item Type: Thesis (Diploma thesis)
Keywords: genotoxicity, Comet assay, induction factor, PLHC-1 cell line, ZFL cell line, EMS, H2O2
Supervisor: Klobučar, Goran
Date: 2010
Number of Pages: 36
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 03 Sep 2014 11:15
Last Modified: 03 Sep 2014 11:15
URI: http://digre.pmf.unizg.hr/id/eprint/2610

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