Comparison of the multiple viral respiratory infections incidence in dependence of the localization and clinical presentation of infection in children

Čižmek, Ines (2010) Comparison of the multiple viral respiratory infections incidence in dependence of the localization and clinical presentation of infection in children. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Acute respiratory tract infection is a leading cause of hospitalization of children, particulary infants younger than one year. This study used a rapid and sensitive method (multiplex PCR assay) to detect 12 different respiratory pathogens including influenza A, influenza B, parainfluenza (PIV) types 1, 2 and 3, respiratory syncytial virus (RSV) groups A and B, adenovirus, human rhinovirus, human metapneumovirus (MPV) and human coronavirus OC43/HKU1 and 229E/NL63 from respiratory specimens of 62 children treated over 3-month period (February-May) for acute respiratory tract infections in the Clinic for children diseases in Zagreb. Viruses were identified in 54 (87.1%) of the children. Influenza A and influenza B viruses were not detected, RSV was detected in 29 (46.8%), PIV in 25 (40.3%), rhinovirus in 17 (27.4%), MPV in nine (14.5%), adenovirus in six (9.7%) and coronaviruses in three (4.8%) of the patients. Coinfections with two or more viruses were observed in 27 (50%) patients. Eighteen patients (60%) infected with RSV had a coinfection, while six out of nine patients tested positive for MPV had a coinfection (2/3). RSV was significantly associated with lower respiratory tract infections (χ²=10.6; p<0.01). Presence of a multiple infection was not significantly associated with severity or localization of the infection (p>0.05). Multiplex PCR assay is more sensitive for diagnosis of the main viruses that cause respiratory tract infections compared with conventional methods and obtains results in a clinically relevant time period. Further investigations are needed to broaden our understanding of the significance of multiple infections.

Item Type: Thesis (Diploma thesis)
Keywords: viral respiratory tract infections, multiplex PCR assay, multiple viral infections, respiratory syncytial virus
Supervisor: Vraneš, Jasmina
Co-supervisor: Matulić, Maja
Date: 2010
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 03 Sep 2014 12:54
Last Modified: 18 Sep 2014 08:27
URI: http://digre.pmf.unizg.hr/id/eprint/2618

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