Production of human papilloma virus E6 and E7 oncoproteins in Escherichia coli

Jerković, Ivana (2010) Production of human papilloma virus E6 and E7 oncoproteins in Escherichia coli. Diploma thesis, Faculty of Science > Department of Biology.

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Human papilloma virus (HPV) belongs to Papillomavirus family. In the 1970s it has been demonstrated that human infection by this virus in women may lead to the development of cervical cancer. The oncogenic properties of HPV are E6 and E7 genes. The aim of this study was the expression of E6 and E7 genes in order to obtain native, productive proteins for further research. Based on the genome sequence of HPV type 16, specific primers for E6 and E7 amplification were designed. Using a vector system the amplicon were transferred into E. coli bacteria where they were expressed. In this study, we have changed the conditions of the expression in order to find the optimal condition. Although the expression was successful, the proteins remained unsoluble under every tested change. Therefore, the alternative approach that we applied was to extract the proteins in denaturating conditions.

Item Type: Thesis (Diploma thesis)
Keywords: HPV virus, oncogenic, cervical cancer, protein E6, protein E7, foreign protein production, E. coli
Supervisor: Grce, Magdalena
Co-supervisor: Matulić, Maja
Date: 2010
Number of Pages: 62
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 03 Sep 2014 13:02
Last Modified: 04 Sep 2014 08:19

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