Genotoxic effect of Rijeka Bay sediments on PLHC-1 fish hepatoma cell culture assessed by the Comet assay

Kralj, Sonja (2010) Genotoxic effect of Rijeka Bay sediments on PLHC-1 fish hepatoma cell culture assessed by the Comet assay. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Numerous toxic anthropogenic pollutants can accumulate in marine sediments and, later on, enter the aquatic organisms and cause toxic effects. Assessment of genotoxic effects of contaminated sediments using cell culture can serve as an early indicator of negative alterations in the environment. In this study, the genotoxicity of sediment samples collected at the six sites of different pollution intensity in the Rijeka Bay was assessed by the Comet assay on PLHC-1 fish hepatoma cell line. PLHC-1 cells were exposed to different concentrations of organic sediment extracts for 24 h. All of the six sediment samples showed a concentration dependent genotoxic effect. In order to determine which group of pollutants is mostly responsible for the observed genotoxicity, the sediment that demonstrated highest genotoxicity was additionally extracted with three solvents which differ by the degree of polarity. Nonpolar solvents (cyclohexane, dichloromethane) revealed higher genotoxicity than the polar solvent (methanol), while the highest genotoxicity was obtained by the sediment extracted using a solvent mixture dichloromethane-methanol which extracts a wide range of pollutants. Comet assay on PLHC-1 cell culture proved to be an advantageous method of sediments genotoxicity assessment.

Item Type: Thesis (Diploma thesis)
Keywords: sediments, genotoxicity, Comet assay, PLHC-1 cell line
Supervisor: Klobučar, Goran
Co-supervisor: Šrut, Maja
Date: 2010
Number of Pages: 42
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Sep 2014 11:00
Last Modified: 04 Sep 2014 11:00
URI: http://digre.pmf.unizg.hr/id/eprint/2626

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