High resolution melting (HRM) analysis of BRAF V600E mutation in sporadic colon cancer

Bulat, Sandra (2010) High resolution melting (HRM) analysis of BRAF V600E mutation in sporadic colon cancer. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Colorectal cancer arises as a consequence of continuous mutation accumulation in a number of oncogenes and tumor suppressor genes. Approximately 50 % of colorectal tumors have an aberrant MAPK signalling. Although mutations of genes involved in MAPK signalling pathway are known for some time, their importance has recently been brought to scientific attention as a consequence of break-troughs in personalized medicine and modern antitumor therapy. Conventional methods of mutation detection are prone to generating false positive or negative results, therefore, in recent years a method of choice for mutation screening is the high resolution melting fluorescent detection of heteroduplexes (HRM). In this study HRM method was introduced in order to detect the most common BRAF gene mutation, V600E in sporadic colon cancer tumors. A total of 120 tumor samples were analyzed and in 6 (5 %) BRAF V600E mutation was detected and confirmed by DNA sequencing. Finally, HRM method has proved itself to be the simplest and faster alternative to conventional methods of mutation detection and can be used for molecular profiling of other tumors where BRAF mutations have an important role.

Item Type: Thesis (Diploma thesis)
Keywords: BRAF, HRM, MAPK signalling pathway, melting curve
Supervisor: Čačev, Tamara
Co-supervisor: Matulić, Maja
Date: 2010
Number of Pages: 32
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Sep 2014 11:16
Last Modified: 04 Sep 2014 11:16
URI: http://digre.pmf.unizg.hr/id/eprint/2628

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