Sinhronization and increasing of mitosis activity of tobacco cells (Nicotiana tabacum L.) in suspension

Čus, Tihana (2010) Sinhronization and increasing of mitosis activity of tobacco cells (Nicotiana tabacum L.) in suspension. Diploma thesis, Faculty of Science > Department of Biology.

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The cell line of tobacco cultivar BY-2 (Nicotiana tabacum L. cv. Bright Yellow No.2) is one of the most commonly used in plant cell biology. The purpose of this study was to follow the intracellular localization of protein of unknown function from maize (ZmMAB1) during the cell cycle. Synchronization of the cell cycle of tobacco BY-2 cells in the liquid medium was achieved by careful selection of initial dilution, degree of differentiation and subculturing period. In the cell culture which was left two weeks without subculturing, cells became differentiated and mature but after resubculturing in fresh medium, they began to divide, and the third day they reach the highest mitotic index. Visualization of microtubules allowed the identification of particular stages of mitosis, and that was achieved by flourescently labeled protein MAP-4. The maize protein ZmMAB1 labeled with red fluorescent protein showed its colocalization with the microtubules during division.

Item Type: Thesis (Diploma thesis)
Keywords: synchronization, mitotic index, Cells Bright Yellow No.2 , cell division
Supervisor: Leljak Levanić, Dunja
Co-supervisor: Juranić, Martina
Date: 2010
Number of Pages: 58
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Sep 2014 11:24
Last Modified: 18 Sep 2014 08:29

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