Impact of immune system on presence of circulating melanoma cells

Marić Brozić, Jasmina (2013) Impact of immune system on presence of circulating melanoma cells . Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Tyrosinase-specific RT-PCR is highly sensitive and specific method for detection of circulating melanoma cells (CMC). Although CMC might be target of tumor-specific immune reaction, correlation between immunocompetence and presence of CMC hasn’t been studied so far. The aim of this study was to investigate if there is an association between the presence of CMC and different parameters of immune status. Blood samples from 131 patients with melanoma were analyzed. The presence of CMC was analyzed by tyrosinase-specific RT-PCR. Proportions of lymphocyte subsets in blood samples as a parameter of immune status were determined by flow-cytometry. Activity of NK cells and phagocytic functions of granulocytes and monocytes were analyzed as functional parameters of immune status. High specificity and low threshold for detection were shown for tyrosinase as a marker for CMC detection by RT-PCR. Patients positive for tyrosinase had significantly shorter survival, which confirms its prognostic value. No association between the presence of CMC and parameters of immune status was found.

Item Type: Thesis (Doctoral thesis)
Keywords: melanoma, immune status, tyrosinase, tumor markers, circulating tumor cells, tumor immunity
Supervisor: Šamija, Ivan
Date: 2013
Number of Pages: 144
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 Jan 2014 12:52
Last Modified: 22 Sep 2014 12:21
URI: http://digre.pmf.unizg.hr/id/eprint/263

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