Antitumor activity of combined application of double stranded RNA and chemotherapeutics

Kirinec, Gabriela (2010) Antitumor activity of combined application of double stranded RNA and chemotherapeutics. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Toll-like receptor 3, as well as other TLRs, plays a key role in innate immunity. Recent studies have shown that TLR3 is expressed on certain types of tumor cells and that its activation stimulates apotosis of tumor cells and inhibits their proliferation. Also it has been found that activation of TLR3 in combination with antitumor therapeutics increases the cytotoxic effect of TLR3 agonist on tumor cells. This leads to the conclusion that the TLR3 agonists can be used as antitumor agents because of their citostatic and cytotoxic effects on tumor cells. In this study, we investigated the effect of TLR3 ligand, polyinosinic:polycytidylic acid (poly(I:C)), in combination with chemotherapeutics 5-fluorouracil and methotrexate on primary and metastatic pharyngeal carcinoma cell lines. The purpose of this study was to examine the possibility of combined application of poly(I:C) and chemotherapeutics as an antitumor therapy. We have found that combined application of poly(I:C) and chemotherapeutics has a significantly greater inhibitory effect on cell growth than the separate treatment with poly(I:C), i.e. poly(I:C) and chemotherapeutics act synergistically. We also found that the inhibitory effect of combined treatment with poly(I:C) and chemotherapeutics is a result of TLR3-dependent apoptosis.

Item Type: Thesis (Diploma thesis)
Keywords: Toll-like receptor 3, poly(I:C), apoptosis, tumor cells, antitumor activity
Supervisor: Pavelić, Jasminka
Date: 2010
Number of Pages: 34
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Sep 2014 11:56
Last Modified: 04 Sep 2014 11:56
URI: http://digre.pmf.unizg.hr/id/eprint/2632

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