Levels of chemical and social oxidative stress in Drosophila melanogaster measured by expression of Green Fluorescent Protein tagged to genes Hsp70 and Hsp22

Butuči, Melina (2010) Levels of chemical and social oxidative stress in Drosophila melanogaster measured by expression of Green Fluorescent Protein tagged to genes Hsp70 and Hsp22. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The free radical theory of aging postulates that the production of intracellular reactive oxygen species (ROS) is the major determinant of the life span. Heat- shock proteins (Hsp) are increasingly being implicated in aging phenotypes and control of life span across species as well. Green Fluorescent Protein (GFP) is frequently used as a reporter of a gene expression. Here, GFP is fused with the hsp70 and hsp22 genes that are known to be expressed during oxidative stress. It is possible to measure the fluorescence / gene expression in the fruit flies. In this work the levels of chemical and social oxidative stress in fruit flies were measured by expression of GFP tagged to the hsp70 and hsp22 genes. The flies are conditioned in two different ways: first, the oxidative stress was caused by hydrogen peroxide; second, the flies were organized in different social groups, in various ratios males to females. Results showed that there are significant effects of the social group and chemical environment in causing oxidative stress, especially in female fruit flies. In general, flies on hydrogen peroxide had higher Hsp expression. However, the actual genotype never came out as an important factor.

Item Type: Thesis (Diploma thesis)
Keywords: GFP, Hsp, ROS, oxidative stress, social environment
Supervisor: Nuzhdin, Sergey
Co-supervisor: Pavlica, Mirjana
Date: 2010
Number of Pages: 54
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 05 Sep 2014 10:28
Last Modified: 18 Sep 2014 08:09
URI: http://digre.pmf.unizg.hr/id/eprint/2641

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