The assessment of DNA damage and repair following exposure to sevoflurane in vivo

Ferenac, Iva (2010) The assessment of DNA damage and repair following exposure to sevoflurane in vivo. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Aim of the study was to investigate the relationship between DNA damage and repair of peripheral blood leucocytes (PBL), liver, kidney and brain cells of Swiss albino mice induced by repeated exposure to sevoflurane. Mice were exposed to sevoflurane in a dose of 2.4vol% for two hours daily, for three consecutive days. Genetic damage of mentioned cells was investigated using comet assay and micronucleus test. To perform comet assay, mice were divided into 5 experimental groups (control group, groups of exposed mice sacrificed third day of experiment immediately after exposure ot sevoflurane, two hours, six hours and twenty-four hours later). The results showed statistically significant increase in mean TL, TM and TI values in all examined organs vs. the control group. The DNA damage at a specific time after the repeated exposure to sevoflurane was different for each examined organ. Significant DNA damage immediately post exposure to sevoflurane was observed in PBL. The induced damage in organs occured 6 hours later than in PBL what was expected according to toxicokinetic of drugs, where blood is the first compartment to absorb the sevoflurane. However none of the assayed tissues revealed signs of repair until 24th hour after the exposure. To distinguish unrepaired genome damage in vivo micronucleus test was applied. Number of micronuclei in reticulocytes showed statistically significant increase compared with control group at all observed times after the treatment.

Item Type: Thesis (Diploma thesis)
Keywords: Comet assay, DNA damage, DNA repair, sevoflurane, inhalation anaesthetics, Micronucleus test, Swiss albino mice
Supervisor: Oršolić, Nada
Date: 2010
Number of Pages: 23
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 05 Sep 2014 11:28
Last Modified: 05 Sep 2014 11:28
URI: http://digre.pmf.unizg.hr/id/eprint/2646

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