Variability and heritability of immunoglobulin G glycosylation

Pučić Baković, Maja (2013) Variability and heritability of immunoglobulin G glycosylation . Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Immunoglobulin G (IgG) is the most abundant glycoprotein in the human plasma and a major effector molecule of the humoral immune response. Glycans are essential structural components of the IgG antibody and even small changes in glycan composition can have a profound influence on IgG effector function by modulating binding to the Fc receptors. A development of the high-throughput IgG purification from plasma and optimization of chromatographic method for IgG glycan analysis of high resolution and sensitivity enabled a first large-scale study of IgG N-glycome in a population. Results from over 2000 individuals revealed a very high variability while heritability of IgG glycans was generally between 30 and 50%. Subclass-specific IgGFc N-glycosylation analysis showed a significant age and sex-dependance. The most prominent changes in glycosylation in females were observed during menopausal age. Age-dependant changes in children differed from changes in adult population in both,direction and intensity. Genome-wide association study (GWAS) of the IgG N-glycome identified nine genetic loci that control IgG glycosylation.

Item Type: Thesis (Doctoral thesis)
Keywords: IgG, N-glycans, HILIC, variability, heritability, aging, gender, IgG subclass specificity, GWAS
Supervisor: Lauc, Gordan
Date: 2013
Number of Pages: 112
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 Jan 2014 13:04
Last Modified: 22 Sep 2014 19:28
URI: http://digre.pmf.unizg.hr/id/eprint/265

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