The influence of coxsackie adenovirus receptor expression on integrin expression in human embryonal rhabdomyosarcoma cell line

Špeljko, Tea (2010) The influence of coxsackie adenovirus receptor expression on integrin expression in human embryonal rhabdomyosarcoma cell line. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Coxsackie adenovirus receptor (CAR) is a component of intercellular junctions, a primary receptor for adenovirus type 5 (Ad5) and a tumor suppressor. Little is known about the influence of increased CAR expression on expression of integrins involved in Ad5 vector transduction, and the information gathered so far is contradictory. The present study demonstrates that augmented CAR expression in clones of human embryonal rhabdomyosarcoma (RD) cell line leads to reduced expression of integrin subunit αv and integrin heterodimers αvβ3 and αvβ5. The aforementioned reduction was recorded on a protein level, using flow cytometry, whereas the mRNA level, measured by semiquantitative RT-PCR, did not vary in RD clones overexpressing CAR as compared to RD cells. By testing the transduction efficacy of RD cells and two RD-CAR transfectants by two recombinant Ad5s with shortened fibers, Ad5FbΔ639 and Ad5FbΔ639RGD4C the negative effect of reduced levels of αvβ3 and αvβ5 on Ad5FbΔ639RGD4C transduction was confirmed. Western blot analysis demonstrates an increase in activation of extracellular signal-regulated kinase (ERK1/2) in RD clones overexpressing CAR as compared to RD cells. These findings are important for understanding the biological function of CAR and reveal that increased expression of CAR reduces integrin αvβ3 and αvβ5 expression, which may have serious effects on adhesion, mobility and/or metastatic potential of cells, but also on the transduction efficacy of adenoviral vectors.

Item Type: Thesis (Diploma thesis)
Keywords: coxsackie adenovirus receptor, integrin, pERK1/2, adenovirus, tumor gene therapy
Supervisor: Ambriović Ristov, Andreja
Date: 2010
Number of Pages: 50
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 05 Sep 2014 11:58
Last Modified: 05 Sep 2014 11:58
URI: http://digre.pmf.unizg.hr/id/eprint/2650

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