Human papillomavirus genotyping

Škara, Ana (2009) Human papillomavirus genotyping. Diploma thesis, Faculty of Science > Department of Biology.

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Human papillomaviruses are a heterogenous group of DNA viruses that have significant role in appearance of benign and malignant neoplasm in multilayer epithelium. The purpose of this work was to analyze a frequency in detection of HPV genotypes related to high-risk and lowrisks of cervix cancer formation in cervical swabs. The specimens were collected from women attending the Outpatient Clinics for Urogenital infections of University Hospital for Infectious Diseases "Dr Fran Mihaljevic". For specimens that were positive on the HPV DNA qualitative test, individual HPV genotypes were determined and their distribution was analysed. Using a Hybrid Capture 2 test, the presence of HPV genotypes associated with high-risk or low-risk for the development of cervical cancer was analysed. Hybridization probes specific for high-risk HPV genotypes (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68), and low-risk genotypes (HPV 6, 11, 42, 43, 44) were used in this study. In specimens that were previously positive by HPV DNA qualitative hybridization test, individual HPV genotypes were identified by combining PCR and reverse hybridization. Following DNA isolation, a part of L1 region of the HPV DNA was amplified by PCR reaction. In the next step, the reverse hybridization and oligonucleotide probes were used. These probes were attached to nitrocellulose membranes. The results of the qualitative analysis of high-risk and low-risk HPV genotypes showed that HPV DNA was detected in 178 of 429 women (41.4%). Individual HPV genotyping showed that HPV-16 and HPV-31 were the most frequent HPV genotypes in analyzed specimens.

Item Type: Thesis (Diploma thesis)
Keywords: human papillomaviruses, cervical cancer, cervical intraepithelial neoplasia, reverse hybridization, polymerase chain reaction
Supervisor: Židovec Lepej, Snježana
Co-supervisor: Oršolić, Nada
Date: 2009
Number of Pages: 35
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Sep 2014 08:46
Last Modified: 17 Sep 2014 13:01

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