Definition of HLA-DRB haplotypes by microsatellite D6S2878 analysis in a Croatian population

Bašić, Marijana (2009) Definition of HLA-DRB haplotypes by microsatellite D6S2878 analysis in a Croatian population. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this study, we analyzed alleles at D6S2878 microsatellite among 53 unrelated subjects in order to define HLA-DRB haplotypes. Number of D6S2878 alleles detected in one haplotype depends on the number of DRB genes (active and pseudogenes) contained by a specific haplotype. Analysis of HLA-DR1, and HLA-DR52 haplotypes revealed two D6S2878 alleles. Shorter allele in HLA-DR1 haplotype (D6S2878-136bp) matched the allele on pseudogene DRB6, while longer matched the alleles on DRB1 locus (D6S2878- 154bp and D6S2878-161bp). In HLA-DR52 haplotype D6S2878 allele which matched alleles on DRB1 locus (172bp-206bp) was shorter than allele which matched DRB3 locus (180bp-214bp). We observed three D6S2878 microsatellite alleles HLA-DR51 and HLADR53 haplotypes. The shortest allele in HLA-DR51 haplotype matched the allele on pseudogene DRB6 (D6S2878-144bp, 152bp, 154bp and 174bp), while the longer alleles (176bp-204pb) corresponded to alleles at DRB1 locus or in the case of alleles of the lenght 180bp-220bp to the alleles at DRB5 locus. Pseudogene DRB7 in HLA-DR53 haplotypes had the shortest D6S2878 allele (D6S2878-135bp), while alleles D6S2878 of the lenght 170bp-194bp matched alleles at DRB1 locus and alleles D6S2878 of the lenght 176bp-182bp matched alleles at DRB4 locus. Allele DRB1*08 displayed only one D6S2878 allele which could be either 170bp or 176bp long.

Item Type: Thesis (Diploma thesis)
Supervisor: Grubić, Zorana
Date: 2009
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Sep 2014 09:58
Last Modified: 09 Sep 2014 09:58
URI: http://digre.pmf.unizg.hr/id/eprint/2669

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